Equipment/Technology
The DNA Sequencing Lab uses the capillary electrophoresis technology developed by Applied Biosystems (ABI) to resolve fluorescently-labeled DNA on the 16-capillary ABI 3130xl Gene Analyzer. The lab offers both DNA sequencing and fragment (microsatellite) analysis to research users. Options include enumeration of polymorphisms (SNPs) in patient samples, a service which complements SNP discovery protocols offered in the Human Genotyping Lab. The lab has computers available for DNA and protein sequence manipulation.
Additional Instrumentation: Applied Biosystems 9600 thermocycler, Savant Speed Vac120 vacuum concentrator
Software: Applied Biosystems Suite – (Sequence Analysis 5.2, GeneMapper 4.0, SeqScape 2.5) Invitrogen Vector NTi10.3 suite, Sequencer 5.0, Geneious 4.0.2.
Service Charges
| DNA Sequencing: | $5/sequencing reaction (MSM), |
| (reaction and electrophoresis) | $6/reaction (non-MSM) |
| Large-user sequencing discount | $4/reaction (MSM) |
| (full 96-well plate) | $5/reaction (non-MSM) |
| DNA Fragment Analysis: | $1.50 per sample (MSM) |
| (electrophoresis only) | $2 per sample (non-MSM) |
Contact Information
Dr. William Roth
DNA Sequencing Core Leader
Phone: (404) 752-1947
Email: wroth@msm.edu
Ms. Qi Yang
Lab Technician
Phone: (404) 752-1861
Email: qiyang@msm.edu