The DNA Sequencing Lab uses the capillary electrophoresis technology developed by Applied Biosystems (ABI) to resolve fluorescently-labeled DNA on the 16-capillary ABI 3130xl Gene Analyzer. The lab offers both DNA sequencing and fragment (microsatellite) analysis to research users. Options include enumeration of polymorphisms (SNPs) in patient samples, a service which complements SNP discovery protocols offered in the Human Genotyping Lab. The lab has computers available for DNA and protein sequence manipulation.
Additional Instrumentation: Applied Biosystems 9600 thermocycler, Savant Speed Vac120 vacuum concentrator
Software: Applied Biosystems Suite – (Sequence Analysis 5.2, GeneMapper 4.0, SeqScape 2.5) Invitrogen Vector NTi10.3 suite, Sequencer 5.0, Geneious 4.0.2.
|DNA Sequencing: ||$5/sequencing reaction (MSM), |
|(reaction and electrophoresis) ||$6/reaction (non-MSM) |
|Large-user sequencing discount ||$4/reaction (MSM) |
|(full 96-well plate) ||$5/reaction (non-MSM) |
|DNA Fragment Analysis: ||$1.50 per sample (MSM) |
|(electrophoresis only) ||$2 per sample (non-MSM) |
Dr. William Roth
DNA Sequencing Core Leader
Phone: (404) 752-1947
Ms. Qi Yang
Phone: (404) 752-1861